TABLE 1.
Steady-state kinetic parameters for nucleotide incorporation opposite the 3′T of the (6-4) TT photoproduct by hPolη
| Template | Template nucleotide | Incoming residue | Vmax (nM/min) | Km(μM) | Vmax/Km | finc |
|---|---|---|---|---|---|---|
| Nondamaged | T | G | 1.4 ± 0.06 | 9.4 ± 2.1 | 0.15 | 9.5 × 10−3 |
| A | 1.1 ± 0.05 | 0.07 ± 0.02 | 15.7 | 1 | ||
| T | 1.1 ± 0.09 | 26 ± 7 | 0.042 | 2.7 × 10−3 | ||
| C | 1.3 ± 0.07 | 53 ± 8 | 0.025 | 1.6 × 10−3 | ||
| (6-4) TT | 3′ T of (6-4) TT photoproduct | G | 0.9 ± 0.02 | 3.2 ± 0.5 | 0.28 | 7.8 |
| A | 0.9 ± 0.005 | 25 ± 0.6 | 0.036 | 1 | ||
| T | 1.0 ± 0.05 | 84 ± 12 | 0.012 | 0.33 | ||
| C | 1.3 ± 0.2 | 89 ± 37 | 0.015 | 0.42 |