Table 2.
Dilutions of primary and secondary antibodies used for immunoblotting detection of proteins involved in hypoxia and p53 activation in human and mouse cancer cell lysates.
| Protein target | Primary antibody | Secondary antibody | ||||||
|---|---|---|---|---|---|---|---|---|
| 1° antibody (clone or catalog) | Host species | Company | Dilution of 1º | 2° antibody | Host species | Company | Dilution of 2º | |
| HIF-1α (Hs) | 610959 | Mouse | BD biosciences | 1: 500 | Anti-mouse HRP | Goat | Dako | 1: 1000 |
| p53 (Hs) | DO-1 | Mouse | In house32 | 1: 3000 | Anti-mouse HRP | Goat | Dako | 1: 2000 |
| p21 (Hs) | 2947 | Rabbit | Cell signaling technology | 1: 1000 | Anti-rabbit HRP | Swine | Dako | 1: 2000 |
| HIF-1α (m) | NB100-479 | Rabbit | Novus biologics | 1: 2000 | Anti-rabbit HRP | Swine | Dako | 1: 2000 |
| p53 (m) | ab246223 | Rabbit | Abcam | 1: 1000 | Anti-rabbit HRP | Swine | Dako | 1: 2000 |
| p21 (m) | ab188224 | Rabbit | Abcam | 1: 1000 | Anti-rabbit HRP | Swine | Dako | 1: 2000 |
HIF-1α hypoxia inducible factor 1α, Hs human, m mouse.