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. 2023 Mar 7;14:1146234. doi: 10.3389/fpls.2023.1146234

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Copyright © 2023 Margolin, Schäfer, Allen, Gers, Woodward, Sutherland, Blumenthal, Meyers, Shaw, Preiser, Strasser, Crispin, Williamson, Rybicki and Chapman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The change in site-specific N-glycan occupancy of plant-produced and mammalian cell-derived spike. (A) Model of the SARS-CoV-2 S glycoprotein in the native-like prefusion conformation generated by Zuzic et al., (2022). Individual N-glycan sites are colored according to the percentage point change in glycan occupancy of plant-derived protein relative to the comparator HEK293F-produced protein and therefore positive and negative values represent a relative increase or decrease in the glycan occupancy each sequon. (B) Bar graph depicting the percentage point changes depicted in panel A. A negative value indicates lower glycan occupancy in the plant-produced spike protein. Sites that could not be resolved are denoted with an asterisk.