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. 2023 Mar 7;14:1099009. doi: 10.3389/fpls.2023.1099009

Table 1.

Summary cal mutant screen.

ID Ac Glc Xyl Ara Sacch # germ # chemo Chemotype M3 BC segregation Mutant
69-3 nd 73 118 135 nd 11 1 High Ara, Low Glc positive undetermined
107-7 nd 100 79 92 nd 12 2 Low Xyl positive undetermined
236-1 nd 386 95 99 nd 12 3 High Glc positive recessive cal1
330-3 98 132 108 99 110 12 5 High Glc positive undetermined
383-10 102 93 99 100 68 11 4 Low Sacch positive recessive cal2
384-4 100 103 101 100 132 9 2 High Sacch positive undetermined
388-3 98 94 97 127 81 10 7 High Ara positive undetermined
499-1 98 89 92 90 72 9 1 Low Saccharification positive recessive cal5
582-2 99 110 99 126 104 12 2 High Ara positive recessive cal7
590-3 105 110 122 124 125 9 4 High Sacch, High Xyl, High Ara positive dominant cal6
613-2 89 nd nd nd 74 11 1 Low Sacch positive recessive cal4
614-4 94 75 112 114 70 11 2 Low Sacch positive undetermined
631-8 92 68 82 87 62 10 2 Low Sacch, Low Glc positive recessive cal3
736-8 94 76 93 96 50 8 1 Low Sacch positive recessive cal8

Mutant outliers whose chemotype was confirmed in the M3. Highlighted in bold are the cal mutants with a monogenic segregation. Wall-bound acetate content (Ac), Saccharification (Sacch) and relative monosaccharide content are shown as percentage of the average value of the screened individuals. Number of germinated plants per M2 (#germ) and number of individuals showing the corresponding chemotype (#chemo) are also indicated. The M3 column indicates, if the chemotype was observed in the progeny after self-crossing of the outlier. Backcross (BC) segregation indicates if the mutant segregation is compatible with a dominant, recessive, or undetermined trait. Mutant column shows the assigned cal name. Full table with all outliers identified in the screen is shown in Supplementary Table 1 .