TABLE 1.

Lack of obvious phenotypic abnormalities associated with XIAP deficiency^a

Cell type	Stimulation	Readout
LN cell	α-CD3 plus or minus α-CD28	[3H]thymidine incorporation
Draining LN cell	KLH immunization and in vitro restimulation	[3H]thymidine incorporation, IL-2 and IL-4 production
Whole mouse	Response to infection with Listeria monocytogenes	CFU in spleen and liver on day 5
	Response to infection with Leishmania major; in vitro restimulation with L. major antigen	Footpad swelling over time; [3H]thymidine incorporation and IFN-γ production
Splenocyte and thymocyte	UV irradiation	% Apoptosis by PI exclusion
	Gamma irradiation	% Apoptosis by PI exclusion
CD4+ CD8+ thymocyte	α-CD3 injected i.p.	% Apoptosis by PI exclusion
Thymocyte	UV, PMA plus ionomycin	JNK activation
Mouse embryonic fibroblast	UV, anisomycin, TNF, heat shock, PMA plus ionomycin	JNK activation

^aVarious assays in which XIAP-deficient mice or cells from these mice responded in a fashion similar to WT littermate controls are listed. Abbreviations: LN, lymph note; KLH, keyhole limpet hemocyanin; IL, interleukin; IFN-γ, gamma interferon; i.p., intraperitoneally; PMA, phorbol myristate acetate; JNK, c-Jun N-terminal kinase.