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. 2023 Mar 7;14:1099623. doi: 10.3389/fmicb.2023.1099623

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Copyright © 2023 Zhang, Yao, Yang, Jiang, Meng, Cao, Zhou, Wang, Yang, Yang, Sun and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation and identification of pathogenic bacteria. (A) Representative plates from undiluted milk samples from healthy cows. (B) Representative plates from undiluted environmental samples. (C) Representative plates from milk samples (diluted 10 times) with mastitis. (D) Gram-positive irregularity rod-shaped bacteria (scale bar is 10 μm). (E,F) Antimicrobial resistance profiles of the Burkholderia contaminans isolates. The round white papers on the petri dish are antibiotic-sensitive tablets (1: Gentamicin, 2: Ciprofloxacin, 3: Tetracycline, 4: penicillin, 5: lincomycin, 6: Cephalosporin, 7: Streptomycin, 8: Amoxicillin). (G) Phylogenetic analysis of B. contaminans. Red fonts indicate B. contaminans isolates in this study.