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. 2023 Mar 7;14:1099623. doi: 10.3389/fmicb.2023.1099623

Figure 2.

Figure 2

Gating strategies to define bovine B cell subsets and the number and the percentage of B cells in two groups. The lymphocytes are chosen with FSC versus SSC (A) and then singlets are gated using FSC-A and FSC-W (B). By outputting CD21, CD21+ (B cells) are identified (C). By outputting IgG versus CD27, IgG+CD27, IgG+CD27+ are identified, respectively (D). (E) Representative dot plot of CD21+ B cells. (F) Absolute number and percentage and of CD21+ B cells in lymphocytes. (G) Relationship of CFU of B. contaminans with the percentage of CD21+ B cells.