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Identification of multifunctional TNF-α, IFN-γ and IL-2T cells in natural B. contaminans infection. (A) Representative plot of TNF-α, IFN-γ and IL-2 secreted by CD4+CD8−, CD8+CD4− and CD8+CD4+ T cells. Boolean gating was applied in order to identify single-, double-, and triple-cytokine-producing T cells. (B) Percentage of CD4+CD8−, CD8+CD4−, and CD8+CD4+ T cells secreting single, dual, and triple cytokines.
