Figure 4.

Food intake under hunger pressure scales water-elicited responses of Nts^LH neurons

(A) Schema of lens and microendoscope placement.

(B) Enclosure with free access to a food (FL), water (WL), and object (OL) location for 10 min per session.

(C) Stimulus selectivity of individual neurons across three states (following ad libitum food access or acute or chronic food restriction). n = 642 neurons.

(D) Proportion of water-responsive neurons. Left: in mice spending a long and a short time at water location (WL). Right: in mice exhibiting fast and slow water approach. n = 853 neurons, 4 mice, 3 imaging days. ^∗∗p = 0.009, ^∗∗∗p < 0.001, Pearson’s χ² test.

(E) Food intake and average activity of water-responsive neurons. n = 203 neurons, 4 mice, 3 imaging days. ^∗p = 0.0273, Pearson’s correlation.

(F) Activity of individual neurons at WL or FL, 4 mice. Insets: acute restriction, n = 283 neurons, ^∗∗p = 0.0037; chronic restriction, n = 293 neurons, ^∗∗∗p < 0.001, Wilcoxon signed-rank test.

(G–I) Water-excited (We) neurons.

(G) Representative Ca²⁺ trace.

(H) Proportion across 3 states. ns, p = 1; ^∗∗∗p < 0.001, Pearson’s χ² test.

(I) Proportion of We neurons and food intake. n = 96 neurons, 4 mice, 3 imaging days. ^∗∗∗p < 0.001, Pearson’s correlation.

(J–L) Water-inhibited (Wi) neurons.

(J) As in (G).

(K) As in (H), n = 107 neurons, ^∗∗p < 0.0079.

(L) As in (I); ns, p = 0.06.

Data shown are mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Figures S5 and S8.