Figure 5.

Nts^LH neurons promote drinking despite hunger pressure

Chemogenetic activation using CNO (1 mg/kg). Control, n = 9; hM3Dq, n = 9 mice.

(A) Water intake. Group: F(1, 48) = 60, ^∗∗∗p < 0.001, ANOVA.

(B) Food intake. Group: F(1, 48) = 20, ^∗∗∗p < 0.001, ANOVA.

(C) Water or food consumption relative to average consumption of control group. ^∗∗p = 0.0049, paired t test.

(D) Food and water intake. ns, p = 0.26; ^∗p = 0.0323, Pearson’s correlation.

(E) Schematic of motion sequencing (MoSeq) approach.

(F) Usage of water seeking or non-water exploration syllables at water location. ns, p = 0.25; ^∗∗p = 0.0078, paired Wilcoxon signed-rank test.

Data shown are mean ± SEM. ns, not significant; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Figure S6.