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. 2002 Feb 1;30(3):823–829. doi: 10.1093/nar/30.3.823

Figure 1.

Figure 1

Purification of nCaRE-B2-binding proteins. (A) EMSA of fractions (1–14) eluted from a nCaRE-B2–Sepharose column with 0.5 NaCl. Lanes with probe alone (free) and control (con) unfractionated HeLa nuclear extracts are indicated. (B) (Left) Coomassie brilliant blue stained PVDF membrane. Lane 1, marker proteins; lane 2, eluted fraction 5. Bands at 66 and 36 kDa are indicated by arrows. (Right) Western analysis of eluted fraction 5 (lane 3) and control HeLa nuclear extract (lane 4) using anti-APE1 antibody. (C) Sequences of three internal peptides from the 66 kDa species and the numbers indicate the positions of the terminal residues in the hnRNP-L polypeptide.