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. Author manuscript; available in PMC: 2023 Mar 21.
Published in final edited form as: Methods Mol Biol. 2016;1411:503–518. doi: 10.1007/978-1-4939-3530-7_31

Fig. 3.

Fig. 3

Single-molecule trajectories and 2D spatial locations of Importin-β1 (Imp β1) in single NPCs [18]. (a) A typical nuclear transport event of Imp β1 molecules from the cytoplasm to the nucleus. First, a single GFP-NPC (green spot) was visualized in the illumination volume. Then, a single fluorescent Imp β1 molecule (red spot) entered the illumination volume, starting in the cytoplasm (C), interacting with the NPC, and entering the nucleus (N). Numbers denote time in milliseconds. (Scale bar, 1 μm.) (b) Single-molecule trajectories of the transport event in (a). Based on the centroid (red dot) and the dimensions of the NPC, the Imp β1 molecule was determined to interact with the NPC from 0.8 to 2.8 ms. (c) Superimposed plots of 1093 spatial localizations of single Imp β1 molecules located primarily within a rectangular area of 240 × 160 nm