Skip to main content
[Preprint]. 2023 Nov 13:2023.02.02.526686. Originally published 2023 Feb 3. [Version 2] doi: 10.1101/2023.02.02.526686

Figure 6. In vitro detection of apoptosis and Ier3 downregulation in sVpr treated 209 mDCT cells.

Figure 6.

(a) Flow cytometry of apoptotic markers (FLICA and PI) on mDCT cells treated with Vpr. Propidium iodide (PI) was used as the live/death discriminator.

(b) Increase (p < 0.001) in PI-labelled nuclei representing apopotic mDCTcells after treatment with either 1ng per well of sVpr or 0.1uM of positive control staurosporine for 24 hours, compared to untreated controls.

(c) The qPCR analysis showed significant downregulation of Ier3 mRNA at 10 ng/ml of Vpr, compared to untreated controls.