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[Preprint]. 2023 Mar 7:2023.03.06.530899. [Version 1] doi: 10.1101/2023.03.06.530899

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Fig. 4 | — a, Schematic overview of the experimental design used to perform chromosome enumeration using Tigerfish probe sets in 46,XY interphase nuclei also labeled with probe sets targeting non-repetitive DNA designed by PaintSHOP. b, Representative images of nuclei labeled with Tigerfish probe sets (magenta) and PaintSHOP probe sets (yellow) targeting intervals on chr22 (top row) or chrX (bottom row). c, Heatmap displaying the observed distribution of Tigerfish and PaintSHOP puncta per nucleus. Images are maximum intensity projections in Z. Scale bars, 10 μm.