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[Preprint]. 2023 Apr 7:2023.03.08.531787. Originally published 2023 Mar 8. [Version 2] doi: 10.1101/2023.03.08.531787

Figure 4: Neutrophil recruitment and chemotaxis is diminished in GSDMD KO mice.

Figure 4:

A,B RNAseq results as in Figure 3 were further analyzed. A Heat map for top differentially regulated genes involved with Response to Virus as defined by GO biological process. B Heat map for top differentially regulated genes involved with neutrophil chemotaxis as defined by GO biological processes. C REACTOME analysis of differentially expressed genes in WT versus GSDMD KO mice identified significant associations with neutrophil degranulation. D ELISA quantification of TNFα, IL-6, IFNβ, IL-1β, or CCL1 levels in lung homogenates of WT and GSDMD KO mice at day 7 post infection. (*p < 0.05, t-test). E Neutrophil infiltration into the lungs of mock and infected mice quantified by flow cytometry (*p < 0.05, two-way ANOVA and Tukey multiple comparisons test). F ELISA quantification of neutrophil elastase levels in lung homogenates of infected WT and GSDMD KO mice at day 7 post infection. (*p < 0.05, **p < 0.01, t-test).