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[Preprint]. 2023 Mar 8:2023.03.08.531785. [Version 1] doi: 10.1101/2023.03.08.531785

Fig. 3: Characterization of the biosynthetic pathway.

Fig. 3:

a, Proposed biosynthetic route for the Dmp modified C-terminus. b, MALDI-TOF mass spectra of IMAC-purified MpaA1 that were co-expressed with the indicated Mpa biosynthetic proteins in E. coli (4, m/z 5872; 5, m/z 5826; 6, m/z 5827; 7, m/z 5855). c, Enlarged MALDI-TOF mass spectra of mpaABCD, mpaABCM, and mpaABC co-expressions in panel b. d, Carbonyl reactivity using aminooxy probe 8. e, MALDI-TOF MS results for the unmodified peptide 4 and ketone intermediate 5 (oxime 9, m/z 5931).