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(A) Citrate synthase activity is a reliable marker of mitochondrial content. In our colon tissue homogenate samples, we saw a significant decrease in citrate synthase activity in males (p≤0.001), but not females. (B) Cardiolipin levels, another marker for mitochondrial content, showed a similar result (p≤0.05). Mitochondrial respiration was normalized to mitochondrial content using citrate synthase activity.
