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. Author manuscript; available in PMC: 2023 Mar 21.
Published in final edited form as: J Med Chem. 2019 Mar 5;62(5):2598–2617. doi: 10.1021/acs.jmedchem.8b01927

Figure 5:

Figure 5:

Comparative uptake and intracellular localization (mitochondria vs. lysosomes) of PSs 1, 3, 5 formulated either in Tween80 or Pluronic127F in UMUC3 and T24 cells. The uptake PSs in Tween (A) and Pluronic (B) after 24 h incubation, and the fluorescence was measured using a CARY fluorimeter. The degree of fluorescence in each cell was directly compared as a measure of uptake. The fluorescence of each cell was measured using an Image Stream. Simultaneously, cells were stained with lysosphere green and mitotracker red to determine the subcellular localization specificity the PSs: (C) PSs 1, 3 & 5 Tween80 formulation in UMUC3, (D) PS 1, 3 & 5 Tween formulation in T24, (E) PSs 1, 3 & 5 Pluronic formulation in UMUC3 and (F) PS 1, 3 & 5 Pluronic formulation in T 24 cell line. The higher bright detail similarity score to a particular site reflects stronger overlap of the PS fluorescence to that site, either lysosomes or the mitochondria.