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. 2002 Feb 1;30(3):775–781. doi: 10.1093/nar/30.3.775

Figure 3.

Figure 3

EMSAs of ASF-1 and in vitro translated TGA2.2, TGA2.1 and TGA1a with a radiolabelled 98 bp as-1 fragment. Numbers above the lanes indicate which oligonucleotide was used as competitor DNA (Fig. 2). For the –2 mutant, the oligonucleotide as-1(–2)GA was used. Quantitative numbers are given in Table 2. For TGA2.2, TGA2.1 and TGA1a, data obtained with oligonucleotides (+4, +6, +8 and +10) are not shown, but values are indicated in Table 2. The binding reaction contained either 5 µg nuclear extract (ASF-1) or 0.5 µl (TGA2.2), 1.0 µl (TGA2.1) or 2 µl (TGA1a) of the respective in vitro translation reactions.