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. 2002 Feb 1;30(3):775–781. doi: 10.1093/nar/30.3.775

Figure 4.

Figure 4

Influence of TGA2.2–VP16 on expression from as-1 and as-1(–2)GA. Mesophyll protoplasts were electroporated with reporter plasmids (10 µg) encoding either the as-1 element (wt), the as-1(–2)GA (–2) element or a mutant as-1 element (m, unable to bind to ASF-1; see Fig. 2) within the –90 region of the CaMV 35S promoter. The amount of the co-electroporated activator plasmid encoding TGA2.2–VP16 under the control of the HBT promoter is given below the columns. The empty HBT vector was used to adjust the total amount of electroporated plasmid DNA to 30 µg. Protoplasts were incubated in the presence of 50 µM auxin. A representative experiment out of three repetitions is shown. Activation of the as-1(–2)GA (–2) element (10 µg) by TGA2.2–VP16 (20 µg) varied between 8- and 24-fold, activation of the wt element varied between 2- and 5-fold.