Figure 9.

Changes in ¹H spectra in the imino proton region upon titration of Rz-3 with MgCl₂. (A) ¹H spectra in the presence of 0, 4 and 10 mM MgCl₂ at 25°C. (B) The chemical shift changes upon addition of 10 mM MgCl₂ are mapped on the secondary structure of Rz-3. Gray, yellow, orange and red colors indicate the chemical shift changes at <0.02, 0.03–0.05, 0.06–0.09 and >0.10 p.p.m., respectively. The residues in open letters are those which do not have imino protons or for which the changes are not measurable due to signal overlap.