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KuA increased the osteoblastic differentiation and mineralized nodule formation of osteoblastic MC3T3-E1 cells. A MDA. B Relative ALP activity. C Alizarin Red SOD. D Relative cell viability. E mRNA level in MC3T3-E1 cells. *P < 0.05, **P < 0.01,***P < 0.001,****P < 0.0001. KuA: Kukoamine A; OVX: ovariotomy; ALP, Alkaline Phosphatase; PYGM, Glycogen Phosphorylase, Muscle Associated; OCN, osteocalcin; Osterix: Sp7 Transcription Factor
