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. 2023 Mar 21;25:45. doi: 10.1186/s13075-023-03028-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — TAK-715 alleviated IL-1β-induced inflammation in NPCs. NPCs were preincubated with TAK-715 for 2 h, and then, IL-1β was added for 48 h. A Western blot analysis was used to analyze COX-2 and HMGB1 in NPCs; B, C COX-2 and HMGB1 immunoblots were quantitatively analyzed. D The fluorescence intensity of COX-2 was analyzed by immunofluorescence assays (scale bar: 20 μm). E ImageJ was used to analyze the fluorescence intensity of COX-2. The data are presented as the mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)