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. 2023 Mar 20;21:98. doi: 10.1186/s12951-023-01855-w

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization and internalization of HUVECs-derived Exos by RAW264.7. A Morphology of Exos and si-Exos identified by TEM. Scale bar = 200 nm. B Western blot analysis of the specific markers of exosomes, including CD63, CD81 and TSG101. C The particle size distribution and particle concentration of Exos and si-Exos detected by NTA. D The uptake of Exos and si-Exos by RAW264.7 cells. FITC-phalloidin (green) and DAPI (blue) were used to stain the cytoskeleton and nucleus of RAW264.7, respectively. Exosomes were labeled with Dil (red). Scale bar = 50 μm