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Micro-CT analysis and histological staining of rat bone reconstruction in vivo. A 3D reconstruction images of calvarial bone at 4 and 12 weeks postoperatively. B Quantitative analysis of BV/TV, Tb. N and Tb. Sp in 3D micro-CT images at 4 and 12 weeks. H&E staining (C) and Masson’s trichrome staining (D). Scale bar = 200 μm. HB host bone; NB new bone. * P < 0.05, ** P < 0.01, *** P < 0.001
