Table 2. Apparent equilibrium dissociation constants for wild-type and mutant MutH proteins.
MutL | Protein | Kd (µM) |
---|---|---|
+ | Wild-type | 4.2 ± 0.7 |
+ | D70A | 48 ± 6 |
+ | E77A | 20 ± 4 |
+ | K79A | 3 ± 0.8 |
– | Wild-type | 57 ± 0.6 |
– | D70A | 83 ± 12 |
– | E77A | 67 ± 10 |
– | K79A | 14 ± 4 |