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. Author manuscript; available in PMC: 2023 Nov 7.
Published in final edited form as: Angew Chem Int Ed Engl. 2022 Oct 7;61(45):e202207661. doi: 10.1002/anie.202207661

Figure 5.

Figure 5.

(A,B) ABC purification of a pyridyltetrazine-tagged POI is only a minor component (<10%) in a mixture of proteins. (C,D) Separation of (C) GFP-pyTz and (D) Trx-pyTz from a mixture with excess, non-tetrazine tagged protein. (E) GFP-pyTz (Lane A) was added to bacterial cell lysate (Lane B) and the combined mixture (Lane C) was ABC purified to recover pure GFP-pyTz (Lane D); samples were treated with TCO-TAMRA and analyzed by SDS-PAGE to identify tetrazine labeled protein by in-gel fluorescence and total protein by coomassie stain.