Table 1. DNA primers used in this study.

Target group	Target gene	Primer	Sequences(5’→3’)	Reference
SFGR*1	gltA	gltA-F	CGAACTTACCGCTATTAGAATG	[17]
		gltA-R	CTTTAAGAGCGATAGCTTCAAG
	rOmpA	rOmpA-F	TGGTGGAGCTCATAAGTTACA	[12]
		rOmpA-R	AGTTACATTTCCTGCACCTAC
Ehrlichia	P28/omp1	conP28-F1	AT[C/T]AGTG[G/C]AAA[A/G]TA[T/C][A/G]T[G/A]CCAA	[18]
		conP28-R1	CAATGG[A/G][T/A]GG[T/C]CC[A/C]AGA[A/G]TAG
		conP28-F2	TTA[G/A]AA[A/G]G[C/T]AAA[C/T]CT[T/G]CCTCC
		conP28-R2	TTCC[T/C]TG[A/G]TA[A/G]G[A/C]AA[T/G]TTTAGG
Anaplasma	P44/msp2	p3726	GCTAAGGAGTTAGCTTATGA	[18]
		p3761	CTGCTCT[T/G]GCCAA(AG)ACCTC
		p4183	CAATAGT[C/T]TTAGCTAGTAACC
		p4257	AGAAGATCATAACAAGCATTG
Borrelia	flaB	flaB-F	GCTGAAGAGCTTGGAATGCAACC	[19]
		flaB-R	TGATCAGTTATCATTCTAATAGCA
	glpQ	glpQ-F	CATACGCTTATGCYTTRGGMGCTGA
		glpQ-R	GCAACCTCTGYCATACCTTCTTSTG
	16S rRNA	16S_RT_F	GCTGTAAACGATGCACACTTGGT	[20]
		16S _RT_R	GGCGGCACACTTAACACGTTAG
		BB_FAM	FAM-TTCGGTACTAACTTTTAGTTAA-NFQ-MGB
		BM_VIC	VIC-CGGTACTAACCTTTCGATTA-NFQ-MGB

*1: SFGR (Spotted fever group rickettsiae).