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. 2002 Feb 1;30(3):685–694. doi: 10.1093/nar/30.3.685

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Spectroscopic characterization of purified recombinant 7kMk protein. (A) UV absorption spectrum of 7kMk protein (0.24 mg/ml) in NB buffer. (B) Fluorescence emission spectra of 7kMk (0.14 mg/ml) acquired at an excitation wavelength of 295 nm in buffers NB (‘NaCl’), 1 M K₂HPO₄/KH₂PO₄ pH 7.2 (‘K-Phos’), GB (‘K-Glu’) and NB supplemented with 6 M guanidine HCl (‘GuHCl’). (C) CD spectrum of 0.78 mg/ml 7kMk in a buffer containing 1 M NaCl, 10 mM NaH₂PO₄/Na₂HPO₄, pH 7.2, obtained with a 0.1 mm path length cuvette.

Spectroscopic characterization of purified recombinant 7kMk protein. (A) UV absorption spectrum of 7kMk protein (0.24 mg/ml) in NB buffer. (B) Fluorescence emission spectra of 7kMk (0.14 mg/ml) acquired at an excitation wavelength of 295 nm in buffers NB (‘NaCl’), 1 M K₂HPO₄/KH₂PO₄ pH 7.2 (‘K-Phos’), GB (‘K-Glu’) and NB supplemented with 6 M guanidine HCl (‘GuHCl’). (C) CD spectrum of 0.78 mg/ml 7kMk in a buffer containing 1 M NaCl, 10 mM NaH₂PO₄/Na₂HPO₄, pH 7.2, obtained with a 0.1 mm path length cuvette.