Table 2. Reverse analysis: scoring known match search ranks in Color Depth Maximum intensity projection (CDM) and PatchPerPixMatch (PPPM) results.

PPPM and CDM search results on nine Hemibrain bodies were scored for the presence of known GAL4 matches from the literature (Schretter et al., 2020; Wang et al., 2020a). Only the top-ranking sample for each line and EM body comparison was considered. Searches were performed across only Phase 140× Gen1 MCFO collection data. vpoDN PPPM median line ranks were 7, 49, >400, and >400. Results for bodies 514850616 and 5813063587 are reformatted from Mais et al., 2021 Figure 9.

Name	Hemibrain body ID	Known-matching lines	PPPM median line rank	CDM median line rank	PPPM % in top 50	CDM % in top 50
pC1e	514850616	13	14	58	69%	46%
pC1d	5813063587	12	28	41	58%	50%
aIPg	645456880	5	6	3	80%	100%
oviDN	550655668	4	70	42	50%	75%
oviDN	519949044	4	95	41	50%	50%
SAG	517587356	2	49	78	50%	0%
SAG	5812981862	2	44	118	50%	0%
vpoDN	5813057864	4	NA	95	50%	50%
DNp13	887195902	3	84	53	33%	33%