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. 2023 Mar 6;12:e80246. doi: 10.7554/eLife.80246

Figure 5. Dual treatment causes ATP leakage but not pore formation.

Figure 5.

(A) S. aureus grown to exponential phase was treated with the indicated compounds for 10 min prior to separating the supernatant from the pellet. ATP measurements were taken using a plate reader and normalized to colony forming unit (CFU). (B) S. aureus grown to exponential phase was loaded with 8 μM of SYTOX Green and aliquoted into a microtiter plate prior to treatment. Membrane permeability was measured (485/522 nm) on a plate reader every 2 min for 28 min. (A–B) Cells were challenged with DMSO (control), nisin (200 IU/ml), or PA (11 μg/ml)±VAN (1, 5, or 10 μg/ml) were added as indicated on the graph. Data represent the mean values from n=2 or 3 biologically independent replicates with three technical replicates each ± SD. Statistical significance was determined by one-way ANOVA with Dunnett’s multiple comparisons test comparing the means of the technical replicates of each condition to PA alone. n.s. and * denote, not significant and p<0.0332, respectively.

Figure 5—source data 1. Related to Figure 5A–B.