Skip to main content
. 2023 Mar 6;12:e80246. doi: 10.7554/eLife.80246

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Strain and strain background (Staphylococcus aureus) HG003; MSSA doi:10.1128/IAI.00088-10 BC1561
Strain and strain background (S. aureus) LAC; CA-MRSA doi:10.1073/pnas.0710217105 BC1684
Strain and strain background (S. aureus) JE2 wild-type doi:10.1128/mBio.00537-12. BC9
Strain and strain background (S. aureus) JE2 floA::Tn doi:10.1128/mBio.00537-12. BC1758 SAUSA300_1533; From NTML, strain NE184
Strain and strain background (S. aureus) JE2 crtM::Tn doi:10.1128/mBio.00537-12. BC440 SAUSA300_2499; From NTML, strain NE1444
Strain and strain background (S. aureus) HG003 ezrA-gfp doi:10.1038/nature25506 BC1552 ezrA-gfp was transduced from ColpSGEzrA-GFP into HG003 background for this work
Strain and strain background (S. aureus) VISA doi:10.1128/AAC.01073-08 SA770; BC1477
Strain and strain background (S. aureus) VRSA doi:10.1128/AAC.01073-08 VRS2; BC1479 Vancomycin-resistant
Strain and strain background (Enterococcus faecalis) VRE doi:10.1128/iai.00425- 15 V583; BC231 Vancomycin-resistant
Strain and strain background (E. faecalis) OG1 ATCC BC524 ATCC 47077
Strain and strain background (Enterococcus faecium) Clinical isolate doi:10.1038/s41564-021-00966-0 BC1540
Strain and strain background (Bacillus subtilis) BS49 doi:10.1371/journal.pgen.1006701 BC209
Strain and strain background (Escherichia coli) MG1655 ATCC BC15 ATCC 47076
Strain and strain background (Staphylococcus epidermidis) CSF41498 doi:10.1128/jb.0194 6-14 BC17
Chemical compound and drug Rhamnolipids AGAE Technologies Cat#R90
Chemical compound and drug Palmitoleic acid Cayman Chemical Cat. #10009871
Chemical compound and drug Linoleic acid Cayman Chemical Cat. #90150
Chemical compound and drug Glycerol monolaurate Cayman Chemical Cat.#28170
Chemical compound and drug Lauric acid Cayman Chemical Cat.#10006626
Chemical compound and drug Adarotene Sigma-Aldrich Cat.#SML2061
Chemical compound and drug Benzyl alcohol ThermoFisher Cat.#A396500
Chemical compound and drug Vancomycin ThermoFisher Cat.#AAJ6279003
Chemical compound and drug Fosfomycin Sigma-Aldrich Cat.#P5396
Chemical compound and drug Oxacillin ThermoFisher Cat.#AC45544005
Chemical compound and drug Nafcillin ThermoFisher Cat.#AC46138001
Chemical compound and drug Bacitracin ThermoFisher Cat.#BP29501
Chemical compound and drug Tarocin A1 Sigma-Aldrich Cat.#SML1677
Chemical compound and drug Daptomycin MedChem Express Cat.#HY-B0108
Chemical compound and drug zaragozic acid Sigma-Aldrich Cat.#z2626
Chemical compound and drug Oritavancin Cayman Chemical Cat.#24091
Chemical compound and drug AsKO Sigma-Aldrich Cat.#A6756
Chemical compound and drug CCCP Cayman Chemical Cat.#25458
Other BODIPY-VAN Invitrogen Cat.#V34850 Fluorescently labeled vancomycin, labeling cell wall, Figure 4
Other Nile red Sigma-Aldrich Cat.#72485 Cell membrane dye, Figure 4
Other DiI-C12 ThermoFisher Cat.#D383 RIF specific dye, Figure 4
Other HADA ThermoFisher Cat.#66475 Dye that stains new cell wall, Figure 7
Other WGA-488 ThermoFisher Cat.#W11261 Dye that stains mature cell wall, Figure 7
Other Sytox green ThermoFisher Cat.#S7020 Cell viability dye, Figure 5
Commercial assay or kit BacTiter-Glo Microbial Viability Kit Promega Cat.#G8232
Software and algorithm Graphpad Prism Graphpad.com Prism 9
Software and algorithm Biorender Biorender.com
Software and algorithm Metamorph 7.10 Molecular Devices Acquisition software
Software and algorithm Autoquant Media cybernetics Version 3.1.3 Deconvolution software
Software and algorithm FIJI ImageJ v1.53q