Figure 4.

Electrophoretic mobility shift assay analysis of GST–AtRrp4p and its N-terminal and C-terminal fragments. The protein species used in gel shift assays and their final concentrations in the binding reactions (in nM) are indicated above the respective panels. The protein–RNA complex and the free oligo(rA)₂₃ probe are indicated as C and FP, respectively. The sharp band appearing below the protein–RNA complex in the right two panels is a conformational variant of the oligo(rA)₂₃ probe; its presence and the relative amount does not correlate with the nature or concentration of the protein used in the binding reaction.