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. 2002 Feb 1;30(3):810–817. doi: 10.1093/nar/30.3.810

Figure 3.

Figure 3

SR proteins and hnRNP H interact with the NRS in vivo in a yeast three-hybrid assay. (A) Summary of results from a three-hybrid screen using NRS 740–797 RNA in tandem (740–797×2) as bait for proteins expressed from a HeLa cDNA library. Positive clones are ordered relative to the number of times they were isolated. SR proteins are indicated in bold. (B) Three-hybrid results using the Gal4 activation domain (Gal4AD) alone or fused to hnRNP H (hnRNP H) and expressed with NRS 740–797×2 in either the sense (+) or antisense (–) orientation. Individual colonies were streaked on medium lacking leucine and uracil to confirm viability of the transformants or on medium lacking histidine to assess RNA-mediated activation of the HIS3 reporter gene. Results shown are representative of four independent colonies selected from each transformation.