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. 2022 Oct 28;4(2):100468. doi: 10.1016/j.xplc.2022.100468

Figure 2.

Figure 2

AtABCG14 displayed efflux transport activities toward multiple CK species in BY-2 cells and Arabidopsis cells.

(A) Quantification of tZR, iP, iPR, cZ, and cZR in BY-2 cells transformed with the 35S::GFP (RCS2) and 35S::GFP-AtABCG14 vectors. BY-2 cells were collected 5 days after transfer to fresh culture medium. Data are means ± SD (n = 4).

(B) Quantification of tZR, iP, iPR, cZ, and cZR in BY-2 cells transformed with 35S::GFP (RCS2) and 35S::GFP-AtABCG14. Cells were collected 5 days after subculture. Data are means ± SD (n = 4).

(C) Quantification of iP, iPR, cZ, and cZR in Arabidopsis cells transformed with 35S::GFP-AtABCG14 and WT cells 5 days after subculture. Data are means ± SD (n = 3).

(D–G) Quantification of the content of iP (D), iPR (E), cZ (F), and cZR (G) in 35S::GFP-AtABCG14 transformed Arabidopsis cells and WT cellscollected 5 days after subculture.

Data are means ± SD (n = 4). ∗P < 0.05, ∗∗P < 0.001, Student’s t-test. tZR, trans-zeatin ribotide; iP, isopentenyladenine; iPR, iP ribotide; cZ, cis-zeatin; cZR, cZ ribotide; G14, AtABCG14; N.D., not detected; FW, fresh weight.