Figure 3.

AtABCG14 displayed different efflux transport activities toward multiple CK species in YPH499 yeast cells.

(A) Immunoblotting of the heterologous expression of GFP-AtABCG14 in YPH499 yeast cells harboring GFP-AtABCG14 using GFP antibody. YPH499 yeast cells transformed with an empty vector were used as a control.

(B and C) Quantification of the isotope-labeled CKs ²H₅-tZ, ²H₅-tZR, ²H₆-iP, ²H₆-iPR, ¹⁵N₄-cZ, or ²H₃-DHZ in YPH499 yeast cells harboring GFP-AtABCG14 or an empty vector after feeding with the respective isotope-labeled CKs (B) or the respective isotope-labeled CKs and vanadate (C) for 20 min. Data are means ± SD (n = 4).

(D) Exported ²H₅-tZ content from YPH499 yeast cells harboring GFP-AtABCG14 or an empty vector after feeding with ²H₅-tZ.

Data are means ± SE (n = 4). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, Student’s t-test.