Figure 5.

Shootward long-distance transport of iP-type CK species is mediated by AtABCG14.

(A and B) Quantification of iP-type CKs in the shoot (A) and root (B) of cypDM and atabcg14 cypDM. The seedlings were grown on half-strength Murashige and Skoog agar medium for 8 days before harvest of shoots and roots.

(C) Translocation of exogenous iP and iPR in atabcg14 cypDM. Roots of 8-DAG cypDM and atabcg14 cypDM seedlings were incubated with 50 nM ²H₆-iP for 1 h. The shoots were harvested for CK quantification.

(D and E) CK concentrations of xylem sap in cypDM, atabcg14 cypDM(D), WT, and atabcg14(E). Xylem sap was collected from 25-DAG plants.

(F–I) Quantification of CK content in the shoots (F), roots (G), apoplast (H), and phloem sap (I) of cypDM/cypDM, atabcg14 cypDM/atabcg14 cypDM, atabcg14 cypDM/cypDM, and cypDM/atabcg14 cypDM plants at 25 DAG.

Data are means ± SD. n = 3 in (A)–(E) and (I), n = 4 in (F)–(H). Groups marked with different letters are significantly different (P < 0.05, ANOVA). ∗P < 0.05, ∗∗P < 0.01 (Student’s t-test).