Table 3.
Significant results of the meta-analyzed EWAS of KORA FF4, TwinsUK and Leiden Longevity Study
| ProbeID | Studies* | Effect- size** | p value FDR | p value Q-statistic | I2*** | Foodgroup | Chr | RefGene name | RefGene group | Relation to CpG Island |
|---|---|---|---|---|---|---|---|---|---|---|
| cg06618277 | K–L–T | − 1.39e-04 | 0.050 | 0.215 | 0.349 [0.000;0.789] | Onions-garlic | 13 | N/A | N/A | N/A |
| cg13970894 | K–L–T | − 4.16e-04 | 0.050 | 0.706 | 0.000 [0.000;0.702] | Onions-garlic | 10 | N/A | N/A | N_Shore |
| cg03046445 | K–L–T | 9.94e-05 | 0.019 | 0.012 | 0.774 [0.266;0.930] | Nuts-seeds | 12 | BHLHE41* | 1stExon* | N_Shore |
| cg05275153 | K–L–T | − 4.39e-05 | 4.96e-05 | 3.14e-06 | 0.921 [0.801;0.969] | Nuts-seeds | 4 | RGS12* | Body* | N/A |
| cg08633290 | K–L–T | − 6.72e-04 | 1.70e-05 | 2.79e-05 | 0.905 [0.748;0.964] | Nuts-seeds | 19 | N/A | N/A | N_Shore |
| cg09418283 | K–L–T | 7.31e-05 | 0.005 | 0.013 | 0.770 [0.252;0.929] | Nuts-seeds | 12 | PAWR* | 1stExon* | Island |
| cg10530560 | K–L–T | − 1.40e-04 | 6.49e-07 | 0.027 | 0.722 [0.060;0.918] | Nuts-seeds | 12 | GLI1* | 5'UTR* | S_Shelf |
| cg11701148 | K–L–T | 2.64e-04 | 0.005 | 0.134 | 0.503 [0.000;0.856] | Nuts-seeds | 8 | MYOM2 | TSS200 | N/A |
| cg12430457 | K–L–T | − 2.00e-04 | 0.047 | 4.47e-04 | 0.870 [0.630;0.955] | Nuts-seeds | 12 | SYT1* | 5'UTR* | N/A |
| cg12611195 | K–L–T | − 1.22e-04 | 0.038 | 9.64e-11 | 0.957 [0.905;0.980] | Nuts-seeds | 6 | PPP1R14C | Body | N/A |
| cg13471114 | K–L–T | 2.21e-04 | 0.036 | 0.190 | 0.397 [0.000;0.814] | Nuts-seeds | 2 | OTX1 | TSS1500 | Island |
| cg14436861 | K–L–T | − 2.70e-04 | 4.96e-05 | 2.89e-07 | 0.934 [0.840;0.972] | Nuts-seeds | 11 | WEE1* | 3'UTR* | N/A |
| cg14828673 | K–L–T | − 1.17e-04 | 0.027 | 0.022 | 0.739 [0.126;0.922] | Nuts-seeds | 8 | TOP1MT | Body | N/A |
| cg15864779 | K–L–T | − 2.77e-05 | 0.005 | 0.995 | 0.000 [0.000;0.000] | Nuts-seeds | 17 | ATP5H* | TSS200* | Island |
| cg16790682 | K–L–T | 8.13e-05 | 0.038 | 0.007 | 0.798 [0.360;0.936] | Nuts-seeds | 12 | PIP4K2C* | TSS200* | Island |
| cg21251785 | K–L–T | − 3.12e-04 | 0.020 | 2.08e-10 | 0.955 [0.901;0.980] | Nuts-seeds | 9 | TRPM3* | TSS1500* | N/A |
| cg23415756 | K–L–T | 5.25e-05 | 0.001 | 0.273 | 0.229 [0.000;0.920] | Nuts-seeds | 17 | NTN1 | Body | Island |
| cg25554998 | K–L–T | − 2.43e-04 | 0.027 | 0.005 | 0.811 [0.411;0.940] | Nuts-seeds | 11 | N/A | N/A | N_Shore |
| cg27344289 | K–L–T | − 2.49e-04 | 1.19e-04 | 0.002 | 0.834 [0.497;0.945] | Nuts-seeds | 5 | FLJ41603 | Body | N/A |
| cg27496650 | K–L–T | 5.30e-05 | 0.008 | 0.363 | 0.012 [0.000;0.897] | Nuts-seeds | 8 | TOX | TSS1500 | Island |
| cg14732699 | K–T | 5.60e-06 | 0.049 | 0.140 | 0.540 [0.000;0.887] | Milk | 8 | MYC | Body | Island |
| cg03846926 | K–L–T | 2.26e-04 | 0.048 | 0.007 | 0.799 [0.363;0.936] | Cream | 10 | C10orf140 | 5'UTR | S_Shore |
| cg06947913 | K–L–T | 2.82e-04 | 0.020 | 3.95e-14 | 0.968 [0.934;0.984] | Cream | 12 | FAIM2 | TSS200 | Island |
| cg08846079 | K–L–T | − 4.73e-04 | 0.044 | 0.518 | 0.000 [0.000;0.842] | Cream | 1 | N/A | N/A | N/A |
| cg09398214 | K–L–T | 1.21e-04 | 0.038 | 5.70e-08 | 0.940 [0.859;0.975] | Cream | 17 | MARCH10* | Body* | Island |
| cg10156125 | K–L–T | 3.86e-04 | 0.020 | 5.53e-11 | 0.958 [0.908;0.981] | Cream | 4 | UGT8* | 5'UTR* | Island |
| cg13331940 | K–L–T | 1.82e-04 | 0.036 | 7.79e-16 | 0.971 [0.943;0.986] | Cream | 15 | MYO1E | Body | Island |
| cg13923646 | K–L–T | − 3.30e-04 | 0.048 | 0.234 | 0.311 [0.000;0.928] | Cream | 1 | N/A | N/A | N/A |
| cg17353893 | K–L–T | − 2.15e-04 | 0.020 | 2.07e-31 | 0.986 [0.975;0.992] | Cream | 7 | CLIP2* | Body* | Island |
| cg22028181 | K–L–T | 1.31e-04 | 0.026 | 7.67e-21 | 0.978 [0.959;0.989] | Cream | 15 | CYFIP1 | TSS200 | Island |
| cg25734572 | K–L–T | 1.46e-04 | 0.036 | 9.21e-05 | 0.892 [0.707;0.960] | Cream | 19 | N/A | N/A | Island |
| cg26633077 | K–L–T | − 6.99e-04 | 0.048 | 5.44e-28 | 0.984 [0.972;0.991] | Cream | 17 | RPTOR* | Body* | S_Shelf |
| cg02488288 | K–L | 3.29e-04 | 0.044 | 0.006 | 0.868 [0.481;0.967] | Plant-oils | 5 | SPRY4* | TSS1500* | N_Shore |
| cg03995571 | K–L | 7.05e-05 | 0.010 | 0.015 | 0.833 [0.303;0.960] | Plant-oils | 8 | FAM49B | TSS1500 | Island |
| cg11189177 | K–L | − 4.92e-04 | 0.044 | 3.95e-04 | 0.920 [0.726;0.977] | Plant-oils | 17 | ABR* | Body* | S_Shore |
| cg18419070 | K–L | − 4.78e-04 | 0.028 | 0.018 | 0.820 [0.239;0.957] | Plant-oils | 3 | SEMA5B | Body | N/A |
| cg02924347 | K–L–T | 6.31e-05 | 0.049 | 0.875 | 0.000 [0.000;0.220] | Butter | 16 | ABCA17P* | TSS1500* | Island |
| cg05781609 | K–L–T | − 2.57e-04 | 0.020 | 2.48e-14 | 0.968 [0.935;0.984] | Butter | 1 | COL24A1 | Body | N/A |
| cg07410571 | K–L–T | 5.11e-05 | 0.049 | 0.718 | 0.000 [0.000;0.685] | Butter | 20 | DDRGK1 | TSS200 | Island |
| cg11798857 | K-L–T | 7.20e-04 | 0.034 | 0.693 | 0.000 [0.000;0.716] | Butter | 20 | FOXA2* | TSS1500* | N_Shore |
| cg11934386 | K–L–T | 4.75e-05 | 2.49e-04 | 5.55e-10 | 0.953 [0.896;0.979] | Butter | 7 | C7orf41* | 1stExon* | Island |
| cg13934553 | K–L–T | − 3.64e-05 | 0.020 | 5.21e-04 | 0.868 [0.621;0.954] | Butter | 1 | PLEKHG5* | Body* | Island |
| cg14046757 | K–L–T | 5.29e-05 | 1.63e-04 | 1.65e-20 | 0.978 [0.959;0.988] | Butter | 14 | ZC3H14* | TSS200* | Island |
| cg14981983 | K–L–T | 3.95e-05 | 0.034 | 1.03e-12 | 0.964 [0.924;0.983] | Butter | 19 | ZNF527 | TSS200 | Island |
| cg18247124 | K–L–T | − 2.85e-04 | 5.45e-04 | 5.87e-12 | 0.961 [0.918;0.982] | Butter | 11 | ASAM | 3'UTR | N/A |
| cg19200140 | K–L–T | − 4.27e-04 | 0.049 | 0.188 | 0.402 [0.000;0.816] | Butter | 20 | N/A | N/A | N_Shelf |
| cg19526600 | K–L–T | 1.70e-04 | 0.034 | 0.825 | 0.000 [0.000;0.461] | Butter | 1 | DIO1* | 1stExon* | N/A |
| cg26351764 | K–L–T | 6.36e-05 | 0.024 | 1.03e-12 | 0.964 [0.924;0.983] | Butter | 20 | FAM83D | TSS200 | Island |
| cg26502414 | K–L–T | 4.70e-05 | 0.025 | 0.798 | 0.000 [0.000;0.538] | Butter | 17 | METT10D | 5'UTR | N_Shore |
UCSC RefGene Name Target gene names from the UCSC database, UCSC RefGene Group Describing CpG position. TSS1500 = 200–1500 bases upstream of the Transcription start site (TSS), 5-UTR Within the 5' untranslated region, between the TSS and the ATG start site; Body = Between the ATG and stop codon, irrespective of the presence of introns, exons, TSS or promoters, 3'UTR Between the stop codon and the poly A signal, Relation to UCSC CpG Island The location of the CpG relative to the CpG Island. Shore = 0–2 kb from Island; Shelf = 2–4 kb from Island, N Upstream (5') of CpG Island, S Downstream (3') of CpG Island [66]
*K is short for KORA FF4 inclusion; L is short for Leiden longevity study inclusion; T is short for TwinsUK inclusion
**Effect sizes are %-methylation change per g/day residual intake
***I2 is reported with 95%-confidence interval in brackets as calculated by the metagen function of the meta package
*After a gene name indicates available splice variants