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. 2023 Mar 8;5:1141618. doi: 10.3389/fgeed.2023.1141618

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Copyright © 2023 Papaioannou, Patsali, Naiisseh, Papasavva, Koniali, Kurita, Nakamura, Christou, Sitarou, Mussolino, Cathomen, Kleanthous and Lederer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Editing efficiency with BE in HUDEP-2 cells. (A) (top) Schematic diagram illustrating the BCL11A target with the DNA-equivalent of the 20-nt gRNA and the 3-nt PAM sequence. The orange arrows show the C bases that were edited. The highlighted rectangle indicates the WGATAR binding motif in BCL11A. (bottom) Annotated EditR-generated plot illustrating the percent area of the signal for each base (A|C|G|T) at the corresponding gRNA position for edited HUDEP-2 cells. The highlighted shape shows the editing window, the orange arrows show the edited C bases and the red boxes display the exact percentage (%) of base substitution in the bulk cell population. (B) Chart showing the % base substitution of C>T after base editing. Each bar shows the editing of the corresponding C base along the gRNA. The data are plotted as mean ± s.d. (n = 3).