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. 2023 Mar 8;5:1141618. doi: 10.3389/fgeed.2023.1141618

FIGURE 3.

FIGURE 3

Functional analysis of BE-modified HUDEP-2 cells. (A) Chromatograms of HPLC analysis for the BCL11A and mock targeting at the last day of HUDEP-2 erythroid differentiation, with the peaks showing globin expression. The α-, β- and γ-globin peaks are labelled, and colored peaks indicate γ-globin induction. (B) Immunoblots of the edited HUDEP-2 cells on days 4 and 9 of erythroid differentiation, detecting the protein expression of α-, β- and γ-globin. Dashed lines indicate where images were simplified by removal of surplus lanes (see Supplementary Figure 2 for the full image). (C) Quantification of HPLC analysis in a chart showing the globin ratios of β/α and (Gγ+Αγ)/α. Data are plotted as mean ± s.d. (n = 2). (D) Quantification of immunoblots in a chart showing the % of (Gγ+Αγ)/α expression in each target on days 4 and 9 of erythroid differentiation. Data are plotted as mean ± s.d. (n = 2).