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Brewer/Fangman 2D gels of transfected plasmid DNA (A) cut with EcoRI and (B) cut with BclI. Both were probed with pUC19. (C) Schematic diagram of the main autoradiographic patterns that can be generated by this technique. The gap in the replication fork arc in B is a blotting artefact. See text for further explanation.
