Table 4.
Comparative analysis functional genes related to N and P metabolism of soil bacteria.
Treatment | NFFA | NFA | DFA | GAN | GAP | |
---|---|---|---|---|---|---|
Spring | L1C1 | 7968 ± 1184.68a | 4146.33 ± 397.81a | 10190 ± 734.72bc | 22304.33 ± 2125.17a | 164247 ± 4734.48a |
L1C2 | 6377.33 ± 1372.51ab | 4053 ± 218.09ab | 10862.67 ± 280.9ab | 21293 ± 2111.14a | 151945.67 ± 6087.11a | |
L1C3 | 3936 ± 145.14c | 3527.33 ± 276.1bc | 9354 ± 193.45c | 16817.33 ± 443.6b | 113045.67 ± 5058.62c | |
L2C1 | 5288.67 ± 1201.81bc | 4360.67 ± 276.8a | 11608.67 ± 658.01a | 21258 ± 4228.28a | 152421.67 ± 15321.09a | |
L2C2 | 3671.67 ± 101.19c | 3374.33 ± 377.31c | 9761.67 ± 307.11bc | 16807.67 ± 1167.53b | 137102 ± 6061.43b | |
L2C3 | 3722.67 ± 248.09c | 3343 ± 340.74c | 9295.67 ± 1164.67c | 16361.33 ± 1054.67b | 112817.67 ± 5730.07c | |
Autumn | L1C1 | 21089 ± 969.35a | 10654.67 ± 589.55a | 22881.33 ± 835.97a | 54625 ± 1747.79a | 397943.33 ± 19602.93a |
L1C2 | 19958.33 ± 1954.3ab | 9289 ± 965.06ab | 20197 ± 2016.25a | 49444.33 ± 3855.53ab | 371200.33 ± 16870.22a | |
L1C3 | 16616 ± 1842.29b | 8918 ± 508.66b | 18578.33 ± 4332.23a | 44112.33 ± 5241.44b | 333575 ± 70090.14ab | |
L2C1 | 20697.33 ± 1091.38a | 8839.67 ± 1253.06b | 21807.33 ± 3075.77a | 51344.33 ± 3767.77ab | 357470.33 ± 9445.11a | |
L2C2 | 19851 ± 3432.89ab | 9644.67 ± 300.9ab | 20517.67 ± 4273.95a | 50013.33 ± 7947.17ab | 337817 ± 73672ab | |
L2C3 | 11662 ± 1285.12a | 6339 ± 444.66c | 16598 ± 3757.27a | 34599 ± 5110.82c | 260776.33 ± 45050.68b | |
F-value | L | 13.273** (35.60) | 16.422** (40.60) | 0.270ns (1.10) | 5.578* (18.90) | 6.685* (21.80) |
C | 31.828** (72.60) | 20.601** (63.20) | 5.375* (30.90) | 18.660** (60.90) | 11.158** (48.20) | |
D | 676.695** (96.60) | 712.845** (96.70) | 156.417** (86.70) | 481.999** (95.30) | 334.246** (93.30) | |
L*C | 0.544ns (4.30) | 3.341* (21.80) | 0.188ns (1.50) | 0.577ns (4.60) | 0.118ns (1.00) | |
L*D | 0.002ns (0.01) | 8.589** (26.40) | 0.396ns (1.60) | 0.654ns (2.70) | 3.183ns (11.70) | |
C*D | 8.023** (40.10) | 6.266** (34.30) | 1.348ns (10.10) | 4.509* (27.30) | 0.881ns (6.80) | |
L*C*D | 5.616** (31.9) | 7.953** (39.90) | 0.593ns (4.70) | 2.513ns (17.30) | 0.549ns (4.40) |
The L represents the micropore group spacing, the C represents the capillary arrangement density, the D represents the different planting seasons of tomato, the data are all average ± standard deviation in the table, the bracketed number is the factor relative contribution%, the same below. The NFFA represents the soil bacterial nitrogen fixation functional gene abundance, the NFA represents the soil bacterial nitrification functional gene abundance, the DFA represents the soil bacterial denitrification functional gene abundance, the GAN represents the soil bacterial nitrogen metabolism functional gene abundance, GAP represents the soil bacterial phosphorus metabolism functional gene abundance. Different letters in the same line meant significant difference at 0.05 level, *: P<0.05, **: P<0.01, ns: P>0.05.