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. 2023 Mar 8;14:1135767. doi: 10.3389/fgene.2023.1135767

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Copyright © 2023 Guo, Mu, Li, Gao, Wang, Li and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of MFN2 activated NLRP3 inflammasome in NP cells. (A) NP cells were transfected as indicated. IL-1β released was assessed by ELISA. (B, C) NP cells were stimulated with MSU. MFN2 mRNA expression was assessed by RT-qPCR and Western blot. (D) Pyroptosis was determined by LDH assay. (E) The Western blot assay for NLRP3, IL-1β cleavage and caspase-1 activation. (F) NP cells were transfected as indicated. NLRP3, pro- IL-1β and pro-caspase-1 mRNA were assessed by RT-qPCR. (G) NP cells were transfected as indicated. IL-1β released was assessed by ELISA.