Fig. 2.

Effect of infectious mimetics and pro-inflammatory stimulus on IL-1α and HMGB1 secretion by SW480 cells. Cells were stimulated with a Poly(I:C), b LPS and c TNF-α for 24 h. IL-1α (left panel) and HMGB1 (right panel) secretion were determined by ELISA. Bars represent mean data (± SEM). One-way ANOVA with Dunnett’s correction was used to determine statistical differences. The levels of IL-1α and HMGB1 in cells treated with Poly(I:C), LPS and TNF-α were compared to unstimulated cells. *P < 0.05