Fig. 6.

Role of NLRP3 inflammasome and caspase-1 in mediating IL-1α and HMGB1 secretion in HT-29 cells. Cells were stimulated for 24 h with Poly(I:C) (100 μg/mL) in the presence or absence of a NLRP3 specific inhibitor; CRID3 and b caspase-1 inhibitor; AC-YVAD-CHO. IL-1α (left panel) and HMGB1 (right panel) concentration were determined by ELISA. c Adherent cells were lysed and the activity of caspase-1 in cell lysates were determined using caspase-1 activity kit. Bars represent mean data (± SEM). One-way ANOVA with Bonferroni’s correction was used to determine statistical differences. IL-1α and HMGB1 levels in cells treated with Poly(I:C) alone were compared to unstimulated cells, and cells treated with Poly(I:C) in the presence of CRID3 or AC-YVAD-CHO. *P < 0.05