Fig. 7.

Effect of IL-1α and the different redox states of HMGB1 on cellular proliferation of HT-29 cells. Cells were stimulated with IL-1α, DS and NO HMGB1. a The proliferation rate of HT-29 cells was measured via MTT at 6, 12, 48 and 72 h and b captured every hour via live imaging (top panel) and the gap closure percentage and rate were graphed (bottom panel). Bars represent mean data (± SEM). One-way ANOVA with Dunnett’s correction was used to determine statistical differences. Cell proliferation of stimulated cells were compared to unstimulated cells. Magnification 4 ×, scale bars, 100 µm (top right). *P < 0.05