Table 1.
Antibodies used for immuno-fluorescence.
| Antibody | host | Fixation | Dilution | Cat. # | Company |
|---|---|---|---|---|---|
| Dectin-1 | Rabbit polyclonal | Methanol | 1:200 | NBP1–25514 | ovus Biologicals |
| Dectin-1 (GE2) | Mouse monoclonal | PFA | 1:50 | ab82888 | Abcam |
| LC3A/B (D3U4C) | Rabbit monoclonal | Methanol | 1:100 | 12741 | Cell Signaling |
| Rubicon | Mouse monoclonal | PFA | 1:100 | ab156052 | Abcam |
| LAMP2 (H4B4) | Mouse monoclonal | Methanol | 1:200 | sc-18822 | Santa cruz |
| Ki67 | Rabbit monoclonal | PFA | 1:400 | RBK027 | Zytomed |
| Muc2 (996/1) | Mouse monoclonal | PFA | 1:200 | ab11197 | Abcam |
| Lysozyme | Rabbit polyclonal | PFA | 1:400 | A 0099 | Dako |
| Dectin-2 | Mouse monoclonal | PFA | 1:100 | ab107572 | Abcam |
| ZO-1/TJP1 | Mouse monoclonal | PFA | 1:100 | 33-9100 | Thermo Scientific |
| EpCAM (VU-1D9) | Mouse monoclonal | PFA or Methanol | 1:400 | 187372 | Abcam |
| EpCAM | Rabbit polyclonal | PFA or Methanol | 1:400 | ab71916 | Abcam |
| anti mouse AF488 | Donkey | 1:1000 | ab150109 | Abcam | |
| anti mouse AF647 | Donkey | 1:1000 | ab216773 | Abcam | |
| anti rabbit AF488 | Donkey | 1:1000 | ab150065 | Abcam | |
| anti rabbit AF647 | Donkey | 1:1000 | ab150067 | Abcam |