Figure 3.

In vitro effect of the isolated components and of assembled new gel formulations on Glioblastoma cells. (a) U87-MG-Red-FLuc cells were treated with cell medium (CTRL), mesoporous SiO₂ nanoparticles (SiO₂) and polycaprolactone microparticles (PCL) for 24 h, causing no significant reduction in viable cells number. (b) U87 MG-Red-FLuc cells were treated with cell medium (CTRL), the empty thermogel (THG), and the thermogels containing mesoporous SiO₂ nanoparticles (THG@SiO₂) and polycaprolactone microparticles (THG@PCL) for 24 h, causing no significant reduction in viable cells number. (c) TMZ-containing SiO₂ nanoparticles and PCL microparticles efficiently release active drug into the cell medium to induce cell death after 24 h treatment in U87 MG-Red-FLuc cells (*p < 0.05). (d) THG@SiO₂-TMZ and THG@PCL-TMZ efficiently release active TMZ into the cell medium to induce cell death after 24 h treatment in U87-MG-Red-FLuc cells (*p < 0.05; **p < 0.005).