Figure 2.

Characterization of histone octamers harboring systematic deletions of the H4 N-terminus. (A) Amino acid sequence of the histone H4 N-terminal ‘tail’ domain. The endpoints of the deletions are indicated. Asterisks indicate the lysine residues that can be acetylated in vivo. (B) Histone octamers were refolded from histones H3, H2A, H2B and a series of H4 variants from which N-terminal amino acids were sequentially deleted. The histones were analyzed as in Figure 1. The identity of the bands is indicated on the left.