Table 1.
TNF induces differential regulation of key cellular pathways in HSCs with implications for survival, reconstitution, and differentiation
| Adj. p value | Combined score | +TNF | Consequence | |
|---|---|---|---|---|
| BioPlanet | ||||
| Rho GTPase cycle | 0.008 | 65.07 | Down | Expression is linked to HSC retention, engraftment, and survival. |
| Protein processing in the endoplasmic reticulum | 1.68e-14 | 347.25 | Up | Low levels of transcription and translation are linked to undifferentiation and dormancy. |
| Gene expression | 7.31e-9 | 67.59 | Up | |
| Protein metabolism | 3.90e-7 | 69.26 | Up | |
| MSigDB Hallmark | ||||
| Interferon gamma response | 5.964e-14 | 239.19 | Up | Increased signaling is associated with apoptosis and reduced reconstitution capacity. |
| Unfolded Protein Response | 4.504e-9 | 162.62 | Up | Responds to accumulation of unfolded/misfolded proteins. Controls cell viability and function. |
| Apoptosis | 0.0002246 | 36.58 | Up | Cell death. |
Enrichment of HSC potency/reconstitution-associated gene sets upon HSC TNF exposure.